I assume by "work" you mean "move". Striders only move on maps with strider nodes. Quoting from the FAQ:

Originally posted by Blixibon:

Make sure the map you're playing on has strider nodes. Most maps on the workshop state whether they have strider nodes, but you could often test it by just seeing if they move when you get out of their sight. You should be spawning your strider where these nodes are available. Also make sure the strider movement setting is enabled. (See Utilities > Enhanced Sandbox NPCs > Settings) You can also try cleaning up or reloading the map. If the strider was moving before but stopped moving, it might have gotten stuck on something and canceled everything. Replace the strider or try typing "ent_fire !picker settargetpath esboxnpcs_striderpath1" in the console while looking at it.

k

I'm having the same issue. I've used the "ai_nodes" command to look at the nodes of a few maps that should work but don't (gm_genesis, gm_bigcity, gm_flatgrass), and I noticed that only construct has nodes in the air, while all the others I checked only had nodes in the ground. Could this be the cause of the issue?

Originally posted by Shorng:

I'm having the same issue. I've used the "ai_nodes" command to look at the nodes of a few maps that should work but don't (gm_genesis, gm_bigcity, gm_flatgrass), and I noticed that only construct has nodes in the air, while all the others I checked only had nodes in the ground. Could this be the cause of the issue?

but that begs the question, is it possible to make a mod that generates air nodes?